个人简介：

龙艳萍，南方科技大学研究副教授。2007年本科毕业于西北农林科技大学，2015年博士毕业于中国科学院遗传与发育生物学研究所，2016年加入南方科技大学生物系翟继先课题组从事博士后研究并工作至今。2022年植物生物学女科学家学术交流会“优秀女青年奖”获奖人。现主要方向为基于高通量测序技术的植物单细胞测序和三代长测序技术的开发和应用以及基因表达调控机制相关研究。主导开发植物单细胞核全长测序技术FlsnRNA-seq、单分子水平全长新生RNA分析方法FLEP-seq以及用于拟南芥基因组多位点相互作用及关联甲基化修饰的Pore-C 技术。目前已以第一作者（含共同）和共同通讯作者在Nature Plants、Nature protocols、PNAS、Genome Biology、Plant Biotechnology Journal和New Phytologist等国际主流学术期刊上发表多篇高水平研究论文。

研究领域：

1. 植物单细胞测序

2. 三代长测序技术的开发和应用

3. 基因表达调控研究

工作经历：

2023至今  南方科技大学 研究副教授

2019 ~ 2023 南方科技大学 研究助理教授

2016 ~ 2019 南方科技大学 博士后

2015 ~ 2016 华大基因研究院 生物技术研究员

学习经历：

2007 ~ 2015 发育生物学，博士，中国科学院遗传与发育生物学研究所

2003 ~ 2007 生物技术，本科，西北农林科技大学

所获荣誉：

2022  中国植物生理与植物分子生物学学会“优秀女青年”

代表文章： 

(*co-first authors, #corresponding author)

1. Zhang K.*, Zhao X.*, Zhao Y., Zhang z., Liu Z., Liu Z., Yu Y., Li Z., Ma Y., Dong Y., Pang X., Jin X., Li N., Liu B., Wendel J., Zhai J., Long Y.#, Wang T.#, Gong L.#. (2023) Cell type-specific cytonuclear coevolution in three allopolyploid plant species. PNAS. 120(40):e2310881120.

2. Mo W.*, Shu Y.*, Liu B. Long Y., Li T., Cao X., Deng X. #, Zhai J.# (2023) Single-molecule targeted accessibility and methylation sequencing of centromeres, telomeres and rDNAs in Arabidopsis. Nature Plants. 9, 1439–1450.

3. Liu Z.*, Yang J.*, Long Y.*, Zhang C.*, Wang D., Zhang X., Dong W., Zhao L., Liu C., Zhai J.#, and Wang E.# (2023) Single-nucleus transcriptomes reveal spatiotemporal symbiotic perception and early response in Medicago. Nature Plants. https://doi.org/10.1038/s41477-023-01524-8

4. Liu Z.*, Kong X.*, Long Y.*, Liu S., Zhang H., Jia J., Cui W., Zhang Z., Song X., Qiu L., Zhai J.#, Yan Z.# (2023) Integrated single-nucleus and spatial transcriptomics captures transitional states in soybean nodule maturation. Nature Plants. https://doi.org/10.1038/s41477-023-01387-z.

5. Qin Y., Long Y., Zhai J.# (2022) Genome-wide characterization of nascent RNA processing in plants. Current Opinion in Plant Biology. 69:102294.

6. Jia J.*, Lu W.*, Liu B., Yu Y., Jin X., Shu Y., Long Y., Zhai J.# (2022) An atlas of plant full-length RNA reveals tissue-specific and evolutionarily-conserved regulation of poly(A) tail length. Nature Plants.  8(9):1118-1126.

7. Li Z.*, Long Y.*, Yu Y., Zhang F., Zhang H., Liu Z., Jia J., Mo W., Tian S. Z., Zheng M., Zhai J.# (2022) Pore-C simultaneously captures genome-wide multi-way chromatin interaction and associated DNA methylation status in Arabidopsis. Plant Biotechnology Journal. 20(6):1009-1011.

8. Yu Y.*, Zhang H.*, Long Y., Shu Y., Zhai J.# (2022) Plant Public RNA-seq Database: a comprehensive online database for expression analysis of ~45 000 plant public RNA-Seq libraries. Plant Biotechnology Journal. 20, 806-808. 

9. Long Y.*, Jia J.*, Mo W., Jin X., Zhai J.# (2021) FLEP-seq: simultaneous detection of RNA polymerase II position, splicing status, polyadenylation site and poly(A) tail length at genome-wide scale by single-molecule nascent RNA sequencing. Nature Protocols. 16, 4355-4381.

10. Long Y.*, Liu Z.*, Jia J.*, Mo W., Fang L., Lu D., Liu B., Zhang H., Chen W., Zhai J.# (2021) FlsnRNA-seq: protoplasting-free full-length single-nucleus RNA profiling in plants. Genome Biology. 22, 66.

11. Mo W.*, Liu B.*, Zhang H., Jin X., Lu D., Yu Y., Liu Y., Jia J., Long Y., Deng X., Cao X., Guo H., Zhai J.# (2021) Landscape of transcription termination in Arabidopsis revealed by single-molecule nascent RNA sequencing. Genome Biology. 22(1):322.

12. Hu D.*, Yu Y.*, Wang C.*, Long Y., Liu Y., Feng L., Lu D., Liu B., Jia J., Xia R., Du J., Zhong X., Gong L., Wang K.#, Zhai J.# (2021) Multiplex CRISPR-Cas9 editing of DNA methyltransferases in rice uncovers a class of non-CG methylation specific for GC-rich regions. The Plant Cell. 33, 2950-2964.

13. Jia J.*, Ji R.*, Li Z., Yu Y., Nakano M., Long Y., Feng L., Qin C., Lu D., Zhan J., Xia R., Meyers BC, Liu B.#, Zhai J.# (2020) Soybean DICER-LIKE2 regulates seed coat color via production of primary 22-nucleotide small interfering RNAs from long inverted repeats. The Plant Cell. 32, 3662-3673.

14. Jia J.*, Long Y.*, Zhang H., Li Z., Liu Z., Zhao Y., Lu D., Jin X., Deng X., Xia R., Cao X., Zhai J.# (2020) Post-transcriptional splicing of nascent RNA contributes to widespread intron retention in plants. Nature Plants 6, 780-788.

15. Long Y.-P.*, Xie D.-J.*, Zhao Y.-Y., Shi D.-Q.#, and Yang W.-C.# (2019) BICELLULAR POLLEN 1 is a modulator of DNA replication and pollen development in Arabidopsis. New Phytologist. doi: 10.1111/nph.15610.

16. Zhang Y.*, Harris C.J.*, Liu Q.*, Liu W., Ausin I., Long Y., Xiao L., Feng L., Chen X., Xie Y., Chen X., Zhan L., Feng S., Li J., Wang H.#, Zhai J.# (2018) Large-scale comparative epigenomics reveals hierarchical regulation of non-CG methylation in Arabidopsis. PNAS. 115, E1069.

17. Fang H., Liu Z., Long Y., Liang Y., Jin Z., Zhang L., Liu D., Li H., Zhai J.# and Pei Y.# (2017) The Ca2+/calmodulin2- binding transcription factor TGA3 elevates LCD expression and H2S production to bolster Cr6+ tolerance in Arabidopsis. The Plant Journal. 91, 1038-1050.

18. Kuo H.Y., Jacobsen E.L., Long Y., Chen X., and Zhai J.# (2017) Characteristics and processing of Pol IV-dependent transcripts in Arabidopsis. Journal of Genetics and Genomics. 44, 3-6.

19. Wang, S.-Q., Shi, D.-Q., Long, Y.-P., Liu, J., and Yang, W.-C.# (2012) GAMETOPHYTE DEFECTIVE 1, a putative subunit of RNases P/MRP, is essential for female gametogenesis and male competence in Arabidopsis. PloS one. 7, e33595.

专利：

1. 新型冠状病毒样颗粒及其制备方法和应用，翟继先，龙艳萍，贾津布，温舰宇，鹿东东，申请号：202110333400.4，发明专利

2. 一种带编码的凝胶微粒及其制备方法和应用，翟继先，肖丽丹，莫伟鹏，龙艳萍，申请号：202010047034.1，发明专利

3. 单细胞基因组测序用的DNA文库的构建方法，翟继先，龙艳萍，肖丽丹，张飞，鹿东东，专利号：ZL 2019 1 1311804.2，授权日：2023-05-05发明专利